• Home
  • Health
  • General
  • Natural Remedies
  • Diet & Weight Loss
  • Fitness
  • Beauty
  • Recipes

The Health Science Journal

Your Personal Health Library




  • About Us
  • Contact Us
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Write For Us

How to Extract Your Protein Samples

August 5, 2019 by Melissa Bell Leave a Comment

It takes a specific method to extract protein samples for studies while protecting the integrity of the proteins. Using the right technique is crucial for the smooth execution of an experiment. Yet many researchers make common mistakes during this process that can negatively impact their molecular screenings. Use this quick guide to successfully extract protein samples for research.

Why Extract Protein Samples?

Extracting protein samples may be necessary for many different studies, tests, or scientific research. One common reason is for molecular screening. In its broadest definition, molecular screening consists of conducting assays for the quantitative or qualitative assessment of a sample. Results of these assessments can offer a clearer understanding of how a protein functions within an organism. Logically, molecular screening and protein analysis has many practical applications in health and medicine: The analysis of protein interactions can guide pharmaceutical drug development, for example.

Extract Protein Samples

Identify Your Study Method

Before you begin protein sample extraction, decide what method you will be using to study the proteins. Identifying your method will determine the preparation and resources you will need to complete your project. Three main types of protein studies exist:

  1. In vivo. The in vivo method looks at proteins within the whole of an organism. The point is usually to see how proteins interact with other parts of the body and with other proteins.

  2. In vitro. In vitro protein studies use purified and isolated proteins analyzed in a controlled environment, such as a lab. These studies may be necessary prior to using a live model, for safer drug testing.

  3. In silico. An in silico study is the newest method of studying proteins. It uses computer simulations to decrease the time and resources necessary to complete a project. In silico studies are common during cell analysis.

All three methods of studying proteins generally require the need to extract protein samples. Extraction takes purifying and then isolating the protein from other components of the cell, which can be a time-consuming process.

Disrupt the Cells

The first step in protein sample extraction is cell lysis, or the breaking down of the cell. Cell lysis typically involves using viral, enzymic, or osmotic elements that compromise the cell’s integrity. Cell lysis breaks open cells to prevent forces that could compromise the sensitive proteins and DNA.

Cell lysis is the most common strategy in blotting to analyze specific proteins, as well as for protein purification. Completing cell lysis for protein extraction takes using a special detergent, called a reagent. Different reagents exist with unique optimizations according to the needs of the researcher. Use the right reagent for your project. Otherwise, you could also physically disrupt the cells.

Extract the Relevant Protein Fraction

Using the appropriate detergent or the less-common physical disruption method, you will be able to solubilize and separate the proteins you need for your study. This is called protein isolation. It is important to use the correct cell lysis method prior to extraction, so that you don’t damage or modify the proteins. If they become unfolded, then you may need to refold the denatured protein. However, many proteins spontaneously renature on their own upon the removal of the reagent through dialysis. After the isolation of the protein, you can carry out extraction for your study.

SOURCES:

http://www.emdmillipore.com/CA/en/life-science-research/protein-sample-preparation/protein-extraction/ZHWb.qB.hNgAAAE_NNF3.Mm0,nav

https://www.thermofisher.com/ca/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-cell-lysis-and-protein-extraction.html

https://www.biochain.com/general/protein-extraction-methods/

http://www.genlantis.com/cell-lysis.html

https://mpkb.org/home/patients/assessing_literature/in_vitro_studies

  • Author
  • Recent Posts
Melissa Bell
Follow me:
Melissa Bell
Melissa Bell has a strong background in nutrition education, fitness and yoga, and experience working on specialized stretching, bodybuilding and weight loss programs. She is actively studying Japanese, doing research and travelling for conferences while taking care of two children.
Melissa Bell
Follow me:
Latest posts by Melissa Bell (see all)
  • Home Care and Management Tips for Mentally Retarded Children - July 1, 2022
  • 10 Natural Ways to Increase Your Bone Density - July 1, 2022
  • Research for Recovery: Delivering Virtual Healthcare Support - June 22, 2022

Share this post:

Share on FacebookShare on PinterestShare on TwitterShare on LinkedInShare on Email

Filed Under: Health

——-

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *




Follow Us on Social Media

Featured Post

  • Ezoic User Review – AdSense vs Ezoic, Analysis After 2 Years with Ezoic
  • Pinterest Account Suspended & Domain Blocked! And How Ezoic Helped Us Get It Back

Latest Review

GenF20 Plus Review: Potentially Dangerous? A Scientific…

—

Fitness

molds-spore

How to Purify Air from Molds

Future Of Dentistry

How Technology Is Overhauling The Future Of Dentistry

Lumbosacral Neuritis – Symptoms and Treatment Options

Controlled Balance – Back and Spine Exercises, Part 7

laptop communications nurse doctor

5 Ways To Improve Public Health

—

Health

breastfeeding

Home Care and Management Tips for Mentally Retarded Children

people-fun-lunch-skeleton

10 Natural Ways to Increase Your Bone Density

Research for Recovery: Delivering Virtual Healthcare Support

Breast cancer foundation

How to Reduce Your Risk of Breast Cancer?

eyes healthy vs diabetic

5 Effective Techniques to Prevent Diabetic Retinopathy in a Post COVID World

Tags

abs addiction back pain bodybuilding cancer children cholesterol depression Diabetes drugs exercises eyes flexibility food foot heart high blood pressure hips home injuries knee lose weight low back pain muscle office pain pain relief plank push-ups relief sciatic senior skin sleep spine squats stress stretch sugar tea teeth tips water workout yoga



Follow Us On Social Media

Copyright © 2020 · The Health Science Journal ·